By Hans G. Drexler
This booklet represents a necessary reference guide for the entire well-characterized leukemia-lymphoma telephone traces at present to be had. It offers crucial evidence, utilizing the succinct and straight forward layout that has made the FactsBooks so well liked by scientists and scientific researchers. Introductory chapters supply historical past and standpoint for culturing malignant hematopoietic (blood forming) telephone strains. those chapters are through over four hundred accomplished person entries. each one mobile line access highlights crucial scientific, immunological, genetic, and sensible beneficial properties and contains a entire directory of references.
* the complete spectrum of malignant cellphone traces from all hematopoietic phone lineages
* sister cellphone strains and correct subclones
* scientific facts: sufferer, prognosis, remedy prestige, and specimen source
* authentication of derivation and availability
* cytogenetic karyotype
* translocations and fusion genes
* receptor gene rearrangements and genetic alterations
* mobile cultures facets: institution, medium, doubling time, growth
* cytochemical profile
* cytokine construction and reaction to cytokines
* proto-oncogene and transcription issue expression/alteration
* sensible good points: differentiation induction, heterotransplantability
* exact detailed features
* key references
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Extra resources for The Leukemia-Lymphoma Cell Line Factsbook
The IGF-1-induced cell proliferation appeared to be restricted to a low oxygen environment and was blocked at high oxygen concentrations2'35. As purified or recombinant factors are expensive, the CM of some malignant human or murine cell lines can be used alternatively. Such cell lines are, for instance, 5637 (an adherent cell line from a patient with bladder carcinoma), Mo-T (HTLV-II transformed T-leukemia cell line), HDLM-3 (Hodgkin's disease-derived cell line), and WEHI-3 (mouse monocytic cell line)1'36-38.
It is important to check for mycoplasma contamination and to indicate whether the cells are EBV+. Sister cell lines and subclones Subclones of any given cell line and sister cell lines must be distinguished and properly presented (Table 2). g. established at diagnosis and relapse of the same patient) may provide unique opportunities to study the molecular mechanisms involved in disease progression and transformation11. Of particular interest also are pairs of cell lines consisting of one cell line with diseased cells and one cell line with normal, albeit EBV-transformed cells from the same patient (which, as a bonus, provide reliable data allowing for subsequent authentication).
A number of completely synthetic media such as RPMI 1640, Minimum Essential Medium (Eagle's MEM or a-MEM), Dulbecco's Modified Eagle's Medium (DMEM), Iscove's Modified Dulbecco's Medium (IMDM), Ham's F-10 and F-12, L15, McCoy's 5A, and others, including several media designed specifically for unique types of leukemia cells, have been used by researchers for establishment and maintenance of cell lines in suspension cultures (Table 12). It appears that no single medium is well suited for the growth of all types of leukemia-lymphoma cells.
The Leukemia-Lymphoma Cell Line Factsbook by Hans G. Drexler