New PDF release: The genus Fusarium,

By Booth C.

ISBN-10: 0851980465

ISBN-13: 9780851980461

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Not only do serum-free media lack the potential confounding serum-effect, but they also do not require repetitive pretesting beyond the initial test since their composition remains the same. 10. This signal amplification step is required for most ELISpot assays. ) Based on the enzyme, there are two choices for this development step: avidin–horseradish peroxidase (HRP) or avidin–alkaline phosphatase (AP) complex. The enzyme determines which substrate can be used. In case of Fluorospot, no enzyme is necessary.

Key words ELISpot, FluoroSpot, Immune monitoring, T cell assays, B cell assays 1 Introduction The ELISpot methodology was first described in 1983 [1]. The key advantages of ELISpot are as follows: (1) It has outstanding sensitivity, enabling the detection and analysis of cells present in even very low frequencies within cell populations, down to only a few cells including single cells [2]. (2) It is a straightforward, easy-to-adapt technology. (3) It is a functional assay. (4) It can be adapted to high throughput.

Therefore, take pictures every 30 min within a period of 3 h of all microchambers occupied by a single E. coli bacterium (see Fig. 4) (EMCCD camera, 4× objective, exposure time 300 ms, gain × 100 excitation: 470 ± 40 nm, emission: 525 ± 50 nm). 20 Simone Stratz and Petra S. Dittrich Fig. 4 Fluorescent images of the same microchamber enclosing the lysate of a single E. coli. The starting time is defined as the point in time the substrate FDG is added. The fluorescent signal inside the microchamber increases over time as the amount of fluorescent product of the enzymatic reaction increases.

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The genus Fusarium, by Booth C.


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