Download e-book for kindle: Renal Cancer. Methods and Protocols by Jack H. Mydlo

By Jack H. Mydlo

ISBN-10: 0896038289

ISBN-13: 9780896038288

ISBN-10: 1592591442

ISBN-13: 9781592591442

Jack H. Mydlo, MD, and a panel of major clinicians and researchers evaluation each point of the newest surgical, clinical, and immunological recommendations that may be utilized in the prognosis and therapy of renal melanoma. those extensively skilled investigators additionally current a pragmatic account in their most sensible uncomplicated examine tools, together with using opposite transcriptase PCR mixed with genomic hybridization, cadherin, and metalloproteinase expression to bare very important elements within the detection, staging, aggressiveness, and therapy of this disorder. Gene treatment, the iteration of monoclonal antibodies, and using interferon alpha, GM-CSF, and IL-6 also are mentioned. complete and pioneering, Renal melanoma: equipment and Protocols bargains urologists, clinical oncologists, laboratory investigators, and pathologists a pragmatic number of the foremost state-of-the-art innovations and remedies for renal melanoma this present day, including a view of the hugely promising way forward for gene treatment.

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Extra resources for Renal Cancer. Methods and Protocols

Sample text

17) and 12 of 12 (100%) Wilms tumor (6 of 6 in Kim et al. (3); 6 of 6 in Yashima et al. (15). In most of these cases (73 of 83, 88%) the adjacent normal renal tissue in the cancer-bearing Telomerase Assay in Renal Cancer 57 Fig. 2. Telomerase role in cellular senescence and immortalization. kidneys were telomerase-negative (4 of 6 in Kim et al. (3); 29 of 35 in Rohde et al. (14); 10 of 12 in Kyo et al. (17); 30 of 30 in Kinoshita et al, (19). In benign renal tumors, none of six angiomyolipomas or four oncocytomas contained telomerase activity (14,23).

And Morin (7,8,33). The highly sensitive and efficient PCR-based TRAP assay was described by Kim et al. in 1994 (3). The TRAP assay permits large-scale analysis of telomerase activity in human cells and tissues. 2. 1. Equipment 1. 2. 3. 4. 5. 6. 7. 8. 9. 0 mm in diameter. Kontes tube with motorized pestles (Fisher Scientific). Refrigerated microcentrifuge. Pipetman, aerosol-resistant tips (RNase-free) and tubes. 5-mL polymerase chain reaction (PCR) tubes. Thermocycler. Vertical gel electrophoresis apparatus and power supply.

1998) Collecting duct carcinoma of kidney. Semin. Diagn. Pathol. 15, 54–67. 55. Dimopoulos, M. , Logothetis, C. , and Ro, J . Collecting duct carcinoma of the kidney. Br. J. Urol. 71, 388–391. 56. Davis, C. , Mostofi, F. , and Sesterhenn, I. A. (1995) Renal medullary carcinoma: the seventh sickle cell nephropathy. Am. J. Surg. Pathol. 19, 1–11. Pathology of Kidney Tumors 51 57. MacLennan, G. , Farrow, G. , and Bostwick, D. G. (1997) Low-grade collecting duct carcinoma of the kidney: report of 13 cases of low-grade mucinous tubulocystic renal carcinoma of of possible collecting duct origin.

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Renal Cancer. Methods and Protocols by Jack H. Mydlo

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