By Daniel I. Chasman
This article deals in-depth views on each point of protein constitution id, evaluation, characterization, and usage, for a transparent figuring out of the variety of protein shapes, diversifications in protein functionality, and structure-based drug layout. The authors conceal a variety of high-throughput applied sciences in addition to computational the way to research protein constructions and residues. A beneficial reference, this ebook displays present developments within the attempt to resolve new buildings coming up from genome projects, info tips on how to discover and establish mistakes within the prediction of protein structural versions, and descriptions demanding situations within the conversion of regimen methods into high-throughput structures.
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Extra info for Protein structure: determination, analysis, and applications for drug discovery
4, left-hand panel), which is depressed to lower temperatures in the presence of high salt and other crystallization agents (18,43). As the temperature rises, micelles become increasingly dehydrated and begin to aggregate into clusters (18,38). When the lower consolute boundary is crossed, the micelle clusters phase out and form a new aqueous, detergent-rich phase (L100 ). Some polymer crystallization agents, like polyethylene glycol (PEG), also radically alter the phase behavior of nonionic detergents (4,18), particularly the alkyl glycosides detergents, such as d-octyl glucoside and -d-decyl maltoside (-OG and C10 M, respectively).
While the chemical modiﬁcations themselves do not necessarily aﬀect crystallization, the heterogeneity they introduce will. Thus, a wise choice of expression systems and expression conditions will dramatically improve the chances of successful crystallization. 6 Lipid Content A question arises about the eﬀect of lipids on crystallization, as the discussion in Section 2 addresses. While heterogeneous native lipids can have adverse eﬀects on crystallization (45), one might ask if crystallization could occur with a homogeneous complex of protein and native lipids or in the presence of pure lipids.
Recent studies have conﬁrmed that heptane-1,2,3-triol addition can alter the apparent micelle size and behavior (25,27,44,86). The crystallization experiments on protein complexes from photosynthetic bacteria still provide the best examples for the use (and drawbacks) of additives in crystallization protocols (88–91). The drawbacks of small-amphiphile use (protein denaturation, irreproducible nucleation, and crystal metastability) are a result of the high concentrations needed to induce crystallization.
Protein structure: determination, analysis, and applications for drug discovery by Daniel I. Chasman