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Extra info for Mammalian Embryo Genomics (Biological Resource Management in Agriculture)
Html). Preimplantation ESTs: stage-specific genes In addition to their use in microarrays, EST collections can give information on the expression levels of each gene by the frequency in which they appear in individual libraries. Using the PCR-based cDNA library construction method described above, we generated cDNA libraries from preimplantation embryos, at various stages from unfertilized eggs to blastocysts, and obtained around 3,000 ESTs from each library . These ESTs were grouped into clusters based on their sequence similarity, and the frequency for each cluster (which represents individual genes) was calculated.
6. Hagen DR, Prather RS, Sims MM, and First NL (1991), Development of one-cell porcine embryos to the blastocyst stage in simple media. J Anim Sci 69: 1147-1150. 7. , (in preparation). 8. , (in preparation). 9. Whitworth KM, Springer,GK, Forrester LJ, Spollen WG, Ries J, Lamberson WR, Murphy CN, Mathialigan N, Didion BA, Green JA, and Prather RS. Developmental expression of over 2,500 genes during pig embryogenesis: An EST project (in preparation). MAMMALIAN EMBRYO GENOMICS – 512003111P/ISBN 92-64-10426-7 © OECD 2003 SYSTEMATIC ANALYSIS OF MOUSE PREIMPLANTATION DEVELOPMENT – 37 Chapter 4 Systematic Analysis of Mouse Preimplantation Development Wency l.
The efficiencies of producing swine embryos in vitro are also poor compared to other livestock species. Typically, approximately 20% of transferred in vitro produced swine embryos develop into live offspring after embryo transfer . The long-term objective of the present study is to establish comprehensive gene expression profiles from the 4 critical stages of porcine embryo development depicted in Figure 1. Development from the 2- to 4-cell stages (day 2 (D2) after insemination) coincides the transition from maternal to embryonic genome expression within the embryo .
Mammalian Embryo Genomics (Biological Resource Management in Agriculture) by Organization for Economic Cooperation &