By Michael P. Sheetz (Eds.)
Quantity fifty five in tools in cellphone Biology is a concise laboratory ebook that emphasizes the tools and applied sciences had to use unmarried polarized laser mild resource that services concurrently as an optical capture and a dual-beam interferometer. * * presents a pragmatic laboratory advisor for ways and applied sciences used with laser tweezers* comprises entire and easy-to-follow protocols
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Extra resources for Laser Tweezers in Cell Biology
The laser beam should be slightly divergent from the turret to the ceiling to put the trap focus close to the camera focal plane. To view the trap in the sample plane, a coverslip mounted on a slide with 1-pm beads in water can be placed on the microscope. Without the IR camera filter in place, there should be enough light reflected from the coverslip water interface to image the reflected laser diffraction pattern. If the reflected laser 2. Basic Laser Tweezers 37 light overdrives the camera, color filters passing the transilumination wavelength or neutral density filters can attenuate the laser light.
Appl. Phys. Lett. 57,26152616. 1. Forces of a Single-Beam Gradient Laser Trap 27 30. , and Wolf, E. (1959). Electromagnetic diffraction in optical systems. 11. Structure of the image field in an aplanatic system. Proc. R. London. A. 253,358-379. 31. Ashkin, A,, and Dziedzic, J. M. (1973). Radiation pressure on a free liquid surface. Phys. Rev. Left. 30, 139-142. 32. , and Inaba, H. (1991). Two-crossing laser beam trapping of dielectric particles using compact laser diodes. ). Technical Digest 10, 280-282.
2”C to melt the butyl stearate. The reduction in melting point probably reflects impurities in the commercial specimen. Test specimens were prepared by heating the butyl stearate to 37°C and placing 2 p1 droplets on coverslips, where droplets quickly solidified. Smaller droplets were generated by incubating coverslips in a 37°C incubator and then “streaking” the droplet with a pipet tip. Microdroplets (-50 p m diameter, 1-3 p m thick) formed along the “streaks,” which were used as test targets.
Laser Tweezers in Cell Biology by Michael P. Sheetz (Eds.)