Get International Review of Cytology, Vol. 176 PDF

By Kwang W. Jeon

ISBN-10: 0080857159

ISBN-13: 9780080857152

ISBN-10: 0123645808

ISBN-13: 9780123645807

International assessment of Cytology provides present advances and finished reports in mobilephone biology-both plant and animal. Articles handle constitution and keep an eye on of gene expression, nucleocytoplasmic interactions, keep an eye on of telephone improvement and differentiation, and cellphone transformation and development. Authored through a number of the ultimate scientists within the box, each one quantity offers updated details and instructions for destiny learn. Key positive aspects * position of Rab GTPases in Membrane site visitors * Lignification in Plant telephone partitions * Cytoskeleton, Membranes, and telephone Wall Interactions within the Pollen Tube of Flowering vegetation * Biology of the Postsynapic Glycine Receptor * cellphone destiny Specification in Ascidian Embryos * Distribution of Na+, K+-ATPase in Insect Photoreceptor Cells

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Extra resources for International Review of Cytology, Vol. 176

Example text

Furthermore, the protein was found enriched in immuno-isolated basolateral post-Golgi transport vesicles. , 1993a), which seems to correspond to the basolateral membrane domain of epithelial cells (Simons et aL, 1992). The peripheral localization of Rab8a was independently reported by Chen et al. (1993), who used stably transfected Swiss 3T3 or Chinese hamster ovary (CHO) cells expressing an epitope-tagged version of the human Rab8a. The protein seemed to concentrate on membrane ruffles and blebs on the cell surface.

Here, a stacked Golgi fraction is immobilized on magnetic beads and the vesicles formed in the presence of cytosol are analyzed.

Function of Rab9 in the Communication between Endosomes and the Golgi Complex The function of Rab9 was first addressed using a cell-free assay that reconstitutes the transport of the CI-MPR from late endosomes to the TGN, measured as the sialylation of endosome-derived CI-MPR in the presence of Golgi-enriched fractions. , 1993). 5-fold, whereas Rab4b and Rab7 showed no effect. Likewise, in intact cells stably transfected with a Rab9 mutant with preferential affinity for GDP, Rab9 S21N, recycling of CI-MPR from late endosomes to the TGN was inhibited (Riederer et aL, 1994), whereas fluid-phase endocytosis was unaffected.

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International Review of Cytology, Vol. 176 by Kwang W. Jeon

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