By Zev Rosenwaks, Paul M. Wassarman
Human Fertility: equipment and Protocols is meant for all practitioners of reproductive drugs and artwork, in addition to for embryologists and reproductive, developmental, mobilephone and molecular biologists and others within the biomedical sciences. the quantity offers straight-forward demeanour most sensible perform ways for overcoming a bunch of fertility demanding situations. Written within the hugely winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols and tips about troubleshooting and averting recognized pitfalls.
Authoritative and cutting-edge,Human Fertility: tools and Protocols aids scientists in carrying on with to check assisted reproductive technologies.
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Additional info for Human Fertility: Methods and Protocols
Eur J Obstet Gynecol Reprod Biol 125:72–78 Miller PB, Soules MR (1996) The usefulness of a urinary LH kit for ovulation prediction during menstrual cycles of normal women. Obstet Gynecol 87:13–17 McGovern PG, Myers ER, Silva S et al (2004) Absence of secretory endometrium after false-positive home urine luteinizing hormone testing. Fertil Steril 82:1273–1277 General Aspects of Fertility and Infertility 39. Robinson JE, Wakelin M, Ellis JE (2007) Increased pregnancy rate with use of the Clearblue Easy fertility monitor.
Flagellar accessory structures including outer dense fibers and fibrous sheath are also important for structural integrity as shown in the infertile Akap4 knockout male mice , although the preimplantation lethality caused by disruption of Odf2 (potentially via disruption of the essential ciliary functions of its alternative spliced product, Cenexin ) prevented the analysis of its roles in the outer dense fiber . Another instance of a sperm motility defect is caused by the failure to remove the excess residual cytoplasm to achieve a streamlined sperm shape and to fully extend the flagellum, such as the Spem1-deficient sperm with bent neck and tail trapped in the cytoplasm remnants ; however, the roles of SPEM1 in cytoplasm removal during spermiation remain to be determined.
At midgestation, the PGCs start migrating along the hind gut to reach the genital ridge. Although the involvement of several adhesion molecules or G-protein-coupled receptor–ligand pairs in PGC migration has been studied in flies and zebra fishes (reviewed in ref. ), the factors required for PGC migration in mammals are less clear. The cytokine ligand–receptor pair of Kit ligand (KITL) and Kit receptor (KIT) were originally identified as two spontaneous mutant loci, steel and W, respectively, in mice for the infertility in both sexes caused by PGC loss [7, 8], and their binding is required specifically for PGC motility .
Human Fertility: Methods and Protocols by Zev Rosenwaks, Paul M. Wassarman