MlklÓS Palkovits (auth.), Alan A. Boulton, Glen B. Baker's General Neurochemical Techniques PDF

By MlklÓS Palkovits (auth.), Alan A. Boulton, Glen B. Baker (eds.)

ISBN-10: 089603075X

ISBN-13: 9780896030756

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Wllllams and Wilkins, Baltimore Manocha S L , Shantha T R , and Bourne G H. q, Oxford Univ. 7flJ’SU~JldlS~ A Cl/tonr’cll~fccto,~lcAt/n> 111Stucotnw Comd~~mks, Inst Blofislca Univ. sohfcd RLVI~OZJI~/4 Irrdmd7rnl Cc/i Groups fru/ll tire Rnf Bralr~ (Hungarian text), Akadkmlal Klad6, Budapest Palkovlts M (1983a) Neuroanatomlcal Techniques, in Brnrjl Prpfufc~ (Kneger D T , Brownstem M J and Martln J , eds), pp 495-545 Wiley, Chlchester Palkovlts M (198313) Stereotaxlc Map, Cytoarchltectomc and Neurochemlcal Summary of the Hypothalamic Nuclei, Rat, m L:rdoCI’IIW System M07~0g70phs OH Pnflwlo~yy of Lnborator I/ A~rmmls(Jones T C , Mohr U , and Hunt R D , eds), pp 316330 Sprmgel-Verlag, Berlin Palkovlts M and Brownstem M J (1983) Mlcrodlssectlon of Blam Areas by the Punch Technque, 111 BJnlrt Mmod7ssrct1on Tcchr7upeS (Cuello A C , ed), pp l-36 Wiley, Chlchester Palkovlts M , Brownstem M , and Saavedra J M (1974) Serotonm content of the brain stem nuclei m the rat Blnrrl Rcs 80, 237-249 Palkovlts M and Jacobowltz D.

Q, Oxford Univ. 7flJ’SU~JldlS~ A Cl/tonr’cll~fccto,~lcAt/n> 111Stucotnw Comd~~mks, Inst Blofislca Univ. sohfcd RLVI~OZJI~/4 Irrdmd7rnl Cc/i Groups fru/ll tire Rnf Bralr~ (Hungarian text), Akadkmlal Klad6, Budapest Palkovlts M (1983a) Neuroanatomlcal Techniques, in Brnrjl Prpfufc~ (Kneger D T , Brownstem M J and Martln J , eds), pp 495-545 Wiley, Chlchester Palkovlts M (198313) Stereotaxlc Map, Cytoarchltectomc and Neurochemlcal Summary of the Hypothalamic Nuclei, Rat, m L:rdoCI’IIW System M07~0g70phs OH Pnflwlo~yy of Lnborator I/ A~rmmls(Jones T C , Mohr U , and Hunt R D , eds), pp 316330 Sprmgel-Verlag, Berlin Palkovlts M and Brownstem M J (1983) Mlcrodlssectlon of Blam Areas by the Punch Technque, 111 BJnlrt Mmod7ssrct1on Tcchr7upeS (Cuello A C , ed), pp l-36 Wiley, Chlchester Palkovlts M , Brownstem M , and Saavedra J M (1974) Serotonm content of the brain stem nuclei m the rat Blnrrl Rcs 80, 237-249 Palkovlts M and Jacobowltz D.

The suspendmg medium has to be isoosmotic, with an osmolarity of about 800 mosmol Such osmolarity 1s achieved with additions of NaCl, KCl, glycme, and urea to sucrose or dextrane solutions Other additions may be chelators (EDTA or EGTA), protease mhibitors, and antioxidant substances The addition of the mhibitor of acetylcholinesterase, eserme, to the suspending medium, to protect against hydrolysis of free acetylcholme, may be desirable In the technique devised by Tashu-o and Stadler (1978), cholinergic synaptic vesicles are purified from electric organ of Torpedo marwzornf~ using a two-step purification on discontmuous and continuous sucrose density gradients.

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General Neurochemical Techniques by MlklÓS Palkovits (auth.), Alan A. Boulton, Glen B. Baker (eds.)


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