By Gérald Thouand, Robert Marks
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Additional info for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3
The binding of FMN and phosphate was found to protect the enzyme from proteolytic degradation . Circular dichroism spectroscopic studies indicated a conformational change around the mobile loop portion . The mobile loop connecting the β7 strand and the α7 helix consists of residues between 262 and 291. This segment is highly conserved among α-subunits and is not found in the β-subunit .
Photochem Photobiol 87(6):1346–1353 61. Xu X, Soutto M, Xie Q, Servick S, Subramanian C, von Arnim AG, Johnson CH (2007) Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues. P Natl Acad Sci USA 104(24):10264–10269 62. Ahn J-M, Kim JH, Kim JH, Gu MB (2010) Randomly distributed arrays of optically coded functional microbeads for toxicity screening and monitoring. Lab Chip 10(20):2695–2701 63. Kim B, Gu M (2005) A multi-channel continuous water toxicity monitoring system: its evaluation and application to water discharged from a power plant.
E. Ionescu Fig. 5 nL) on a non-modiﬁed glass slide. Printing (upper 2 rows) and 24 h incubation at 30 °C were followed by the removal of non-adhered cells (2 bottom rows) (a). Strain SM111 (1 nL) was printed on APTES-coated glass (5 left columns) and incubated for 24 h at 4 °C, followed by the removal of non-adhered cells (5 right columns) (b). Epifluorescence microscope images of strains SM110 (c) and SM111 (d) were printed (2 nL) on APTES-coated glass. Reprinted with permission from , copyright 2011 RSC obvious modiﬁcation of the bacterial bioluminescent spectrum could be observed (Fig.
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand, Robert Marks